CST, 76701SF, Ikaros (D10E5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Ikaros. Validated for WB,IF,F,ELISA+. Highly specific and rigorously validated in-house, Ikaros (D10E5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #76701) is ready to ship. Product Usage Information This product is the carrier free version of product #9034. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9034 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Ikaros (D10E5) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Ikaros protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg439 of human Ikaros protein. Background The Ikaros family of zinc-finger DNA-binding proteins belongs to the Kruppel transcription factor superfamily. Ikaros proteins are characterized by the presence of an amino-terminal zinc finger DNA-binding domain and a carboxy-terminal dimerization domain. Members of the Ikaros family include Ikaros, Aiolos, Helios, EOS, and Pegasus (1). All family members can form homodimers and heterodimers with other members of the Ikaros family. Most also contain multiple isoforms that are generated as a result of differential splicing, with some isoforms behaving in a dominant negative manner upon dimerization (2). Ikaros (IKZF1, LYF1) is the prototypical Ikaros family zinc-finger transcription factor and is expressed abundantly in lymphoid cells. Genetic studies in mice demonstrate that Ikaros is a tumor suppressor that is important for the normal development of B, T, natural killer, and dendritic cells (3,4). Additional studies show that imbalanced expression of different Ikaros isoforms, as well as mutations in the corresponding gene, can be associated with a number of hematologic malignancies in humans (2,5,6). Alternate Names CLL-associated antigen KW-6; CVID13; DNA-binding protein Ikaros; hIk-1; Hs.54452; IK1; IKAROS; Ikaros (zinc finger protein); IKAROS family zinc finger 1; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; IKZF1; LyF-1; LYF1; Lymphoid transcription factor LyF-1; PPP1R92; PRO0758; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros); ZNFN1A1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG