CST, 76703T, RGS14 (E4N2Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RGS14 mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, RGS14 (E4N2Q) Rabbit Monoclonal Antibody (CST #76703) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:3200 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74575 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity RGS14 (E4N2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total RGS14 protein. Non-specific staining in fixed frozen mouse kidney may be observed by immunofluorescence. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse RGS14 protein. Background Regulator of G-protein signaling (RGS) proteins are a structurally diverse family of more than 30 member proteins enriched in brain that typically limit neurotransmitter receptor- and G protein-linked signaling in the CNS and elsewhere by serving as GTPase-activating proteins (GAPs) (1). RGS14 is a multifunctional signaling protein that regulates post-synaptic plasticity in neurons and acts as a scaffolding protein that integrates G protein, Ras/ERK, and calcium/calmodulin signaling pathways essential for spine plasticity and cell signaling (2). It is highly enriched within pyramidal cells of hippocampal area CA2, allowing RGS14 to naturally suppress long-term potentiation (LTP) and structural plasticity in that region (2-4). RGS14 has been shown to directly interact with two key Ca -activated proteins in CA2 neurons: calcium/calmodulin and CaMKII (5). Alternate Names rap1/rap2 interacting protein; RAP1/RAP2-interacting protein; Regulator of G-protein signaling 14; RGS14; RPIP1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG