CST, 76755SF, CD86 (E2G8P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD86. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, CD86 (E2G8P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #76755) is ready to ship. Product Usage Information This product is the carrier free version of product #91882. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 91882 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD86 (E2G8P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD86 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro239 of human CD86 protein. Background CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9). Alternate Names Activation B7-2 antigen; B-lymphocyte activation antigen B7-2; B7-2; B7-2 antigen; B7.2; B70; BU63; CD28 antigen ligand 2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 molecule; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60-85 Source/Isotype: Rabbit IgG