CST, 76798S, ALAD (E8Z7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ALAD. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ALAD (E8Z7Y) Rabbit Monoclonal Antibody (CST #76798) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ALAD (E8Z7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALAD protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human ALAD protein. Background Delta-aminolevulinic acid dehydratase (ALAD) catalyzes the second step in heme biosynthesis. ALAD is also an endogenous inhibitor of the 26S proteasome (1). Defects in ALAD cause acute hepatic porphyria, an inherited defect in heme biosynthesis in the liver (2). ALAD activity is inhibited by lead, and defects in ALAD result in sensitivity to lead poisoning (3). Reduced expression of ALAD is associated with poor prognosis in breast cancer patients. Overexpression of ALAD in breast cancer cells negatively regulates proliferation, invasion, and transforming growth factor-β (TGF-β)-mediated epithelial-mesenchymal transition (EMT) (4). Alternate Names ALAD; ALADH; aminolevulinate dehydratase; aminolevulinate, delta-, dehydratase; Delta-aminolevulinic acid dehydratase; HEM2; MGC5057; PBGS; Porphobilinogen synthase; testicular tissue protein Li 95 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG