CST, 76828S, MHC Class I (E8E7N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying H2-D1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MHC Class I (E8E7N) Rabbit Monoclonal Antibody (CST #76828) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 65494 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity MHC Class I (E8E7N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MHC Class I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu254 of mouse H2-D1 protein. Background The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8 T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4 T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2). Alternate Names H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H-2D cell surface glycoprotein; H-2D(B); H2-D; H2-D1; H2-DC1-beta transplantation antigen protein; H2-K1; HA11; histocompatibility 2, D region locus 1; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG