CST, 76851S, NADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NADK iso3. Validated for Western Blotting. Highly specific and rigorously validated in-house, NADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody (CST #76851) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NADK isoform 3 protein (UniProt #O95544-3). This antibody does not cross-react with other NADK isoforms. This antibody cross-reacts with a 125 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile49 of human NADK isoform 3 protein (Uniprot #O95544-3). Background NADK, also known as NAD kinase, is a cytoplasmic protein responsible for maintaining the pool of available NADP and NADPH within the cell (1). Using ATP as a phosphate donor, NADK catalyzes the phosphorylation of NAD to NADP . This molecule is then reduced to NADPH and utilized in various metabolic and biosynthetic pathways (2). NADK has been suggested to play a role in glucose metabolism due to the effect NADPH production has on both the insulin secretion and survival of pancreatic β-cells (3). NADPH has a vital role in protecting cells from oxidative stress through its neutralizing effect on reactive oxygen species (ROS), which also accumulate during cell growth (2-4). Along with the p53 tumor suppression protein, NADK has been a suggested target in cancer therapy due to its link to NADPH production and its resulting protective role on growing and proliferating cells (2). Cytoplasmic NADK isoform 3 is transcribed from a unique start site compared to isoforms 1 and 2, and lacks the N-terminal, Akt-dependent, activating phosphorylation sites of Ser44, Ser46, and Ser48 found in the other isoforms (5). Cells engineered to exclusively express NADK isoform 3 exhibit constitutively increased NAD kinase activity, insensitivity to AKT agonists, and increased synthesis of NADP (5). NADK isoform 3 is uniquely upregulated in metastatic nodules of a PDX model of melanoma (6). Isoform 3 expression is driven by transcription factor NRF2 in response to oxidative stress, allowing melanoma cells to survive both oxidative insult and metastatic cascade by increasing NADP synthesis (6). Alternate Names dJ283E3.1; NAD kinase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG