CST, 76883S, CREB (48H2) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying CREB. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CREB (48H2) Mouse Chimeric Monoclonal Antibody (CST #76883) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity CREB (48H2) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total CREB-1 protein. This antibody does not cross-react with other ATF/CREB family members. Non-specific staining of components along the retinotectal pathway was observed by immunofluorescence in fixed frozen mouse tissue. Non-specific signal is observed in formaldehyde fixed frozen mouse retina by immunofluorescence. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification This recombinant chimeric antibody is engineered from CREB (48H2) Rabbit mAb #9197 according to animal-free protocols. This chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondary antibodies are required to detect rabbit-host primary antibodies. The parent antibody, CREB (48H2) Rabbit mAb #9197, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CREB-1 protein. The epitope has been mapped to residues surrounding Glu21. Background CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca , and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). Alternate Names active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a