CST, 76977T, SPTLC1 (F7Y7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPTLC1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SPTLC1 (F7Y7S) Rabbit Monoclonal Antibody (CST #76977) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SPTLC1 (F7Y7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPTLC1 protein. This antibody does not cross-react with SPTLC2 or SPTLC3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys207 of human SPTLC1 protein. Background The serine palmitoyltransferase (SPT) complex catalyzes the initial and rate-limiting step of sphingolipid biosynthesis. This protein complex consists of an SPTLC1/SPTLC2 heterodimer or an SPTLC1/SPTLC3 heterodimer as the catalytic core (1-3). Studies show that mutations leading to unregulated SPT activity and increased sphingolipid synthesis cause amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disease (4). In addition, mutations in that change the substrate of SPT from serine to alanine drive the accumulation of deoxysphingolipids and cause neuropathy. Furthermore, dietary restriction of serine and glycine also leads to the accumulation of deoxysphingolipids in tumors and reduces tumor growth in mouse xenograft models (5). Alternate Names HSAN; HSAN1; HSN1; LBC1; LCB 1; LCB1; Long chain base biosynthesis protein 1; MGC14645; serine C-palmitoyltransferase; Serine palmitoyltransferase 1; serine palmitoyltransferase long chain base subunit 1; serine palmitoyltransferase, long chain base subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTC1; SPTI; SPTLC1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG