CST, 76998S, XRCC1 (E4A3V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XRCC1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, XRCC1 (E4A3V) Rabbit Monoclonal Antibody (CST #76998) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74875 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity XRCC1 (E4A3V) Rabbit Monoclonal Antibody recognizes endogenous levels of total XRCC1 protein. In some cell lysates, this antibody detects a 62 kDa band of unknown origin by western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro623 of human XRCC1 protein. Background The X-ray repair cross-complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8). Alternate Names DNA repair protein XRCC1; RCC; SCAR26; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross complementing 1; X-ray repair cross complementing protein 1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster; XRCC1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG