CST, 77024S, Alpha-internexin Antibody

Polyclonal Antibody for studying INA. Validated for Western Blotting. Highly specific and rigorously validated in-house, Alpha-internexin Antibody (CST #77024) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Alpha-internexin Antibody recognizes endogenous levels of total Alpha-internexin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Alha-internexin protein. Antibodies are purified by peptide affinity chromatography. Background Alpha-internexin is a class-IV neuronal intermediate filament that is involved in morphogenesis of neurons and is localized mostly in synaptic vesicles, primarily found in the post-synaptic compartment where it modulates neurotransmission function by interacting with different neurotransmitter receptors (1,2). Alpha-internexin interacts with tubulin and actin, suggesting its role in axonal transport and stabilization of dendrites (2-4). Expression of Alpha-internexin occurs in embryonic stages in rat at E10 in the cortex, auditory ganglion, olfactory epithelial, spinal cord and brainstem, and expression also occurs in postnatal stages in the cerebellum in mice and humans (3). Alpha-internexin expression is altered in different neurological diseases and disorders; where it decreases in bipolar disorder and increases in schizophrenia and Alzheimer's disease (4-6). Some of these findings were generated by proteomic analysis from Alzheimer's disease and control brains (4). The overexpression of Alpha-internexin enhances the neurite outgrowth during neuronal growth factor induction (7), induces the activation caspase-3, which triggers apoptosis and eventually neuronal death (8). Alpha-internexin could be playing a role in drug addiction, where chronic cocaine exposure decreases the levels of the protein, but not with morphine exposure, suggesting the possibility to interact with different receptors (5,9). Alternate Names 66 kDa neurofilament protein; AINX; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein alpha; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; Neurofilament 5; neurofilament 5 (66kD); Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; TXBP-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 62-67 SOURCE: Rabbit