CST, 77082S, p67phox (F1W6T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p67phox. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, p67phox (F1W6T) Rabbit Monoclonal Antibody (CST #77082) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity p67phox (F1W6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total p67phox protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p67phox protein. Background p67phox is a component of the phagocytic NADPH oxidase complex, which is composed of membrane-bound p91phox and p22phox heterodimers (also known as cytochrome b558), the cytosolic proteins p40phox, p47phox, and p67phox, and a small GTPase, Rac1 or Rac2 (1). The phagocytic NADPH oxidase complex transfers electrons from NADPH to oxygen to produce reactive oxygen species (ROS), which are released by neutrophils and macrophages to combat pathogens (2,3). A phosphorylation cascade drives NADPH oxidase complex activation, and p67phox contributes to this cascade by regulating phosphorylation of several sites on p47phox (4). In chronic granulomatous disease (CGD), in which patients have mutations in phagocytic NADPH oxidase complex genes, this cascade is disrupted, and the capacity of macrophages and neutrophils to produce ROS is severely impaired (5,6). Alternate Names 67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; FLJ93058; NADPH oxidase activator 2; NCF-2; NCF2; Neutrophil cytosol factor 2; neutrophil cytosolic factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADPH oxidase factor 2; NOXA2; p67-phox; P67PHOX Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 67 Source/Isotype: Rabbit IgG