CST, 77215S, ITK (E4X7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Itk. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, ITK (E4X7M) Rabbit Monoclonal Antibody (CST #77215) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24573 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ITK (E4X7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total ITK protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ITK protein. Background Interleukin-2 inducible T cell kinase (Itk, Emt, or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk, and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain, and an SH3 and SH2 domain followed by a carboxy-terminal kinase domain (1). Tec, Rlk, and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that Itk functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates Itk through phosphorylation in the conserved activation loop at Tyr511, and furthermore, Itk is autophosphorylated in the SH3 domain at Tyr180. Itk-Y180F is still capable of phosphorylating PLCgamma1 in contrast to Itk-Y511F, which has lost that function (2-3). Itk -/- mice show reduced lung inflammation, eosinophil infiltration, and mucous production in response to allergic asthma induction. Thus, Itk could become a desirable target for anti-asthmatic treatments (4). Alternate Names EMT; homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; IL2 inducible T cell kinase; IL2-inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; ITK; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK2; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG