CST, 77349SF, beta3-Tubulin (E9F3E) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying tubulin beta-3. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, beta3-Tubulin (E9F3E) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #77349) is ready to ship. Product Usage Information This product is the carrier free version of product #45058. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 45058 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity beta3-Tubulin (E9F3E) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β3-tubulin protein. Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5). Alternate Names Beta III Tubulin; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; MC1R; TBB3; TUBB3; TUBB4; tubulin beta 3 class III; Tubulin beta-3 chain; tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; tubulin, beta, 4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Mouse IgG1 kappa