CST, 77428S, WAPL (D9J1U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WAPL. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, WAPL (D9J1U) Rabbit Monoclonal Antibody (CST #77428) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WAPL (D9J1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total WAPL protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human WAPL protein. Background The cohesin protein complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). WAPL, also wings apart-like or WAPAL, is a chromatin-associated protein that binds to the cohesin protein complex and regulates its function and its interaction with chromatin (3,4). WAPL, through its interaction with cohesin, is important in the regulation of chromosome structure, cell cycle progression and appropriate chromosome segregation (5,6). Depletion of WAPL leads to segregation errors and DNA damage, and in p53 deficient cells leads to aneuploidy (6). Alternate Names FOE; friend of EBNA2 (Epstein-Barr virus nuclear protein 2); Friend of EBNA2 protein; KIAA0261; WAPAL; WAPL; WAPL cohesin release factor; wings apart-like homolog (Drosophila); Wings apart-like protein homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG