CST, 77478SF, MAGE-A10 (E9V2Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAGE-A10. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MAGE-A10 (E9V2Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #77478) is ready to ship. Product Usage Information This product is the carrier free version of product #81740. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81740 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MAGE-A10 (E9V2Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MAGE-A10 protein. This antibody does not cross-react with other MAGE-A proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro323 of human MAGE-A10 protein. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). Melanoma-associated antigen-A10 (MAGE-A10) is a CTA that belongs to the type I MAGE family of proteins. Unlike other members of the MAGE-A subfamily, MAGE-A10 is a nuclear protein (4). Research studies have shown that MAGE-A10 expression is normally restricted to the human testis and placenta but is aberrantly upregulated in a number of human solid tumors, such as lung cancer, melanoma, bladder cancer, and esophageal carcinoma (4-6). Due to its upregulated expression in human tumors and high degree of immunogenicity, MAGE-A10 has received significant attention as a novel immunotherapy target through the use of vaccines and adoptive cell therapy (7-9). Alternate Names Cancer/testis antigen 1.10; cancer/testis antigen family 1, member 10; CT1.10; MAGAA; MAGE family member A10; MAGE-10 antigen; MAGE-A10; MAGE10; MAGEA10; melanoma antigen family A, 10; melanoma antigen family A10; Melanoma-associated antigen 10; MGC10599 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG