CST, 77510S, IRG1 (D6H2Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRG1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IRG1 (D6H2Y) Rabbit Monoclonal Antibody (CST #77510) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IRG1 (D6H2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRG1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly460 of human IRG1 protein. Background IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6). Alternate Names ACOD1; Aconitate decarboxylase; Aconitate decarboxylase 1; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; Immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog; immunoresponsive 1 homolog (mouse); IRG1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG