CST, 77513SF, Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IRF-7 (Ser437/Ser438) phosphate mouse. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #77513) is ready to ship. Product Usage Information This product is the carrier free version of product #24129. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 24129 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of mouse IRF-7 protein when dually phosphorylated at Ser437 and Ser438. This antibody has a preference for Ser437 and can recognize single phosphorylation at this site. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser437/438 of mosue IRF-7 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11). Alternate Names Interferon regulatory factor 7; IRF-7; Irf7; OTTMUSP00000029373 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG