CST, 77576S, CD33 Antibody

Polyclonal Antibody for studying CD33. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Highly specific and rigorously validated in-house, CD33 Antibody (CST #77576) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD33 Antibody recognizes endogenous levels of total CD33 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD33 protein. Antibodies are purified by peptide affinity chromatography. Background CD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin 3 (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands, CD33 transduces inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular functions such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion (1-3). Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML) (4,5). CD33 may also be involved in Alzheimer's Disease (AD) (6-8). Alternate Names CD33; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3; SIGLEC3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-80 SOURCE: Rabbit