CST, 77657S, Raet1 (F4V7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Raet1c mouse/Raet1a mouse/Raet1b mouse/Raet1d mouse/Raet1e mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Raet1 (F4V7S) Rabbit Monoclonal Antibody (CST #77657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Raet1 (F4V7S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Raet1 protein. A non-specific band at 100 kDa can be observed in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His33 of mouse Raet1 protein. Background The activating receptor natural killer group 2, member D (NKG2D) is involved in both innate and adaptive immune response, and functions as a switch determining the activation status of natural killer (NK) cells (1,2). NKG2D binds to a diverse family of ligand molecules, which are expressed at low levels in most cells but can be upregulated by cellular stress response (1-3). Retinoic acid early transcript 1 (Raet1) is a GPI-linked cell surface protein that functions as a ligand for NKG2D, and has been shown to activate NK and T cells (3-5). In addition, Raet1 promotes tumor immune surveillance by inducing proliferation of anti-tumor cytotoxic T lymphocytes (3). Raet1 has been shown to be regulated by the STING/cGAS pathway (5), is involved in response to neuronal damage and encephalitis (6,8), and has been implicated in chronic inflammation and autoimmune disease (7,8). The NKG2D-Raet1 interaction is considered a promising target for immune-based therapies due to the specific expression of stress-induced ligands exclusively on tumor cells and strongly induced NK cell activation (9). Alternate Names MGC118478; Rae; RAE-; Rae-1 alpha; Rae-1 beta; Rae-1 epsilon; RAE-1-alpha; RAE-1-beta; RAE-1-delta; RAE-1-epsilon; RAE-1-gamma; RAE-1alpha; RAE-1b; RAE-1beta; RAE-1d; RAE-1delta; RAE-1g; RAE-1gamma; RAE1A; Rae1alpha; RAE1B; RAE1C; RAE1D; RAE1E; Raet1; Raet1a; Raet1b; Raet1c; Raet1d; Raet1e; retinoic acid early transcript 1, alpha; retinoic acid early transcript 1D; retinoic acid early transcript 1E; retinoic acid early transcript beta; retinoic acid early transcript delta; retinoic acid early transcript gamma; Retinoic acid early-inducible protein 1-alpha; Retinoic acid early-inducible protein 1-beta; Retinoic acid early-inducible protein 1-delta; Retinoic acid early-inducible protein 1-epsilon; Retinoic acid early-inducible protein 1-gamma Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-60 Source/Isotype: Rabbit IgG