CST, 77693S, CLCA1 (E4Y1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CLCA1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CLCA1 (E4Y1J) Rabbit Monoclonal Antibody (CST #77693) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CLCA1 (E4Y1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLCA1 protein. This antibody also recognizes the 45-60 kDa autoproteolytically cleaved form of CLCA1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala745 of human CLCA1 protein. Background Many epithelial surfaces of the body are covered with protective mucus, and disrupted mucus homeostasis has been implicated in increased susceptibility to infection, along with diseases such as colitis, cystic fibrosis, chronic obstructive lung disease, asthma, and colon and lung cancers (1-4). The chloride channel calcium-activated (CLCA) family are secreted proteins that regulate chloride transport and mucin expression (2). CLCAs control the production of mucus in lung, colon, and other tissues (2,3). CLCA1, the first characterized human CLCA family member, is mainly expressed in the lungs and lower digestive tract, particularly intestinal crypt cells (1,2,6,7). CLCA1 has been linked to the pathogenesis of asthma and chronic obstructive pulmonary disease (5), in addition to colitis and colon cancer (1,3,6). CLCA1 expression is significantly altered in the tissues of these patients, and mutations in CLCA1 are associated with increased disease incidence (5,6). CLCA1 contains a consensus proteolytic cleavage site, centered at glycine 694, which is recognized by a metalloprotease domain located within its N terminus (2). Alternate Names CACC; CaCC-1; CACC1; Calcium-activated chloride channel family member 1; Calcium-activated chloride channel protein 1; Calcium-activated chloride channel regulator 1; calcium-dependent chloride channel-1; chloride channel accessory 1; chloride channel regulator 1; chloride channel, calcium activated, family member 1; CLCA family member 1, chloride channel regulator; CLCA1; CLCRG1; FLJ95147; GOB5; hCaCC-1; hCLCA1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-60, 130 Source/Isotype: Rabbit IgG