CST, 77726T, PTPRJ/DEP-1 (E8R1V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTPRJ. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PTPRJ/DEP-1 (E8R1V) Rabbit Monoclonal Antibody (CST #77726) is ready to ship. Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PTPRJ/DEP-1 (E8R1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPRJ/DEP-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp440 of human PTPRJ/DEP-1 protein. Background Protein-tyrosine phosphatase receptor type J (PTPRJ), also known as density-enhanced phosphatase-1 (DEP-1), is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ/DEP-1 functions as a tumor suppressor, and its expression is lost in multiple types of human cancers (7,8). Alternate Names CD148; CD148 antigen; Density-enhanced phosphatase 1; DEP-1; DEP1; HPTP eta; HPTPeta; human density enhanced phosphatase-1; protein tyrosine phosphatase receptor type J; protein tyrosine phosphatase, receptor type, J; protein tyrosine phosphatase, receptor type, J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; R-PTP-eta; R-PTP-J; Receptor-type tyrosine-protein phosphatase eta; SCC1; susceptibility to colon cancer 1, mouse, homolog of Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 240 Source/Isotype: Rabbit IgG