CST, 77814T, PRPF6 (F9A2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PRPF6. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PRPF6 (F9A2R) Rabbit Monoclonal Antibody (CST #77814) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PRPF6 (F9A2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRPF6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro202 of human PRPF6 protein. Background PRPF6 is a critical spliceosomal protein that forms a bridge between the U5 and U4/U6 small nuclear ribonucleoproteins (snRNPs) (1,2). It is phosphorylated by PRP4 kinase along with another PRPF protein of similar function, PRPF31 (3,4). The phosphorylation of these two proteins is a critical step in the stable U4/U5/U6 tri-snRNP complex (5). mutations have been described in retinitis pigmentosa, an autosomal-dominant disease that leads to retinal degeneration (6). PRPF6 has been implicated in cancer through its control of alternative splicing of key oncogenic isoforms, such as the ZAK kinase (7). PRPF6 has also been shown to interact with the androgen receptor (AR) and the AR splice variant 7 (AR-V7), thereby increasing AR transcriptional activity (8). Alternate Names androgen receptor N-terminal domain transactivating protein-1; Androgen receptor N-terminal domain-transactivating protein 1; ANT-1; ANT1; C20orf14; hPrp6; p102 U5 small nuclear ribonucleoprotein particle-binding protein; pre-mRNA processing factor 6; Pre-mRNA-processing factor 6; Prp6; PRP6 homolog; PRP6 pre-mRNA processing factor 6 homolog; PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae); PRPF6; putative mitochondrial outer membrane protein import receptor; RP60; SNRNP102; TOM; U5 snRNP-associated 102 kDa protein; U5-102 kDa protein; U5-102K Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 106 Source/Isotype: Rabbit IgG