CST, 77860S, SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SASH3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody (CST #77860) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SASH3/SLY (F5S3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SASH3/SLY protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro53 of human SASH3/SLY protein. Background Encoded by an X-linked gene, sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY), is a putative lymphoid-specific signaling adaptor (1). One of the first clues that SASH3 plays a role in regulating the biology of lymphoid cells arose from research studies demonstrating that SASH3 is modified by phosphorylation subsequent to T cell receptor (TCR) engagement (2). Furthermore, the presence of SAM and SH3 domains strongly suggests that SASH3/SLY participates in protein-protein interactions to regulate immune cell effector functions downstream of antigen receptor engagement. Indeed, naturally occurring loss-of-function mutations have been identified in human SASH3/SLY that blunt activation of multiple key signaling nodes following TCR engagement, leading to an impairment of T cell proliferation and survival (3). Alternate Names 753P9; CXorf9; HACS2; SAM and SH3 domain containing 3; SAM and SH3 domain-containing protein 3; SASH3; SH3 protein expressed in lymphocytes homolog; SH3D6C; SLY Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG