CST, 77865SF, Ghrelin (D4K1W) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Ghrelin mouse. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Ghrelin (D4K1W) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #77865) is ready to ship. Product Usage Information This product is the carrier free version of product #31865. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 31865 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Ghrelin (D4K1W) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ghrelin protein. This antibody detects acylated (n-octanoyl) and non-acylated (desacyl) forms of ghrelin (confirmed by Western blot analysis of synthetic ghrelin protein). Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse ghrelin protein. Background Ghrelin, also known as appetite-regulating hormone, is a neuropeptide hormone belonging to the motilin family. It is the ligand for the growth hormone secretagogue receptor type 1 (GHS-R), expressed by cells in the hypothalamic ventromedial nucleus and arcuate nucleus (1). Ghrelin is synthesized as a preprohormone by ghrelinergic cells in the gastrointestinal tract; proteolytic cleavage yields a 28-amino acid peptide hormone, which undergoes obligate n-octanoylation at serine 3 by the enzyme ghrelin O-acetyltransferase (GOAT) (2). Binding of n-octanoyl ghrelin to GHS-R stimulates growth hormone release, while simultaneously exerting multiple neuroendocrine affects that influence appetite, gastric motility and energy balance (3). Alternate Names 2210006E23Rik; Appetite-regulating hormone; Ghr; Ghrelin; Ghrl; Growth hormone secretagogue; Growth hormone-releasing peptide; m46; Motilin-related peptide; Mtlrp; MTLRPAP; Obestatin; preproghrelin; proghrelin; Protein M46 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG