CST, 77891S, TBP (D5C9H) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying TBP. Validated for Western Blotting. Highly specific and rigorously validated in-house, TBP (D5C9H) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #77891) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TBP (D5C9H) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total TBP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TBP protein. Background TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex. Alternate Names GTF2D; GTF2D1; HDL4; MGC117320; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TBP; TF2D; TFIID; Transcription initiation factor TFIID TBP subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35-45 Source/Isotype: Rabbit IgG