CST, 77934T, TRPC3 (D4P5S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRPC3 iso3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TRPC3 (D4P5S) Rabbit Monoclonal Antibody (CST #77934) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity TRPC3 (D4P5S) Rabbit Monoclonal Antibody recognizes endogeneous levels of total TRPC3 protein. This antibody does not cross-react with TRPC6 or TRPC7 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TRPC3 protein. Background Transient Receptor Potential Canonical 3 (TRPC3) belongs to the superfamily of TRP cation channels. The TRPC subfamily (TRPC1-7) is a group of calcium-permeable cation channels that mediates the increase in intracellular [Ca2+] following activation by G-protein-coupled receptors or receptor tyrosine kinases (1). TRPC3 is mainly expressed in the brain and various other tissues, though at a much lower level (2). In the brain, TRPC3 is involved in BDNF-induced axonal growth cone plasticity, dendritic spine formation, and neuronal survival (3-6). It is also required for synaptic transmission and motor coordination (7). Outside of the central nervous system, TRPC3 also exerts other important biological functions such as regulating cardiac and vascular contractility, maintaining Ca2+ homeostasis in primary T cells and endothelial cells (8-10). TRPC3 is activated by diacylglycerol and Inositol 1,4,5-trisphosphate (11,12). It is also activated by internal calcium store depletion and regulates mitochondrial calcium uptake (13,14). Alternate Names hTrp-3; hTrp3; SCA41; Short transient receptor potential channel 3; transient receptor potential cation channel subfamily C member 3; transient receptor potential cation channel subfamily C member 3 variant c; transient receptor potential cation channel, subfamily C, member 3; transient receptor potential channel 3; Transient receptor protein 3; TRP-3; TRP3; TRPC3; TRPC3 iso3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG