CST, 78063SF, CHOP (L63F7) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CHOP. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, CHOP (L63F7) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #78063) is ready to ship. Product Usage Information This product is the carrier free version of product #2895. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2895 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CHOP (L63F7) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total CHOP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CHOP protein. Background CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). Alternate Names AltDDIT3; alternative DDIT3 protein; C/EBP homologous protein; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; C/EBPzeta; CCAAT/enhancer-binding protein homologous protein; CEBPZ; CHOP; CHOP-10; CHOP10; DDIT-3; DDIT3; DNA damage inducible transcript 3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth arrest and DNA damage-inducible protein GADD153; growth arrest- and DNA damage-inducible; MGC4154 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Mouse IgG2a