CST, 78128T, MCRIP1 (D2Y8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MCRIP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MCRIP1 (D2Y8V) Rabbit Monoclonal Antibody (CST #78128) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MCRIP1 (D2Y8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total MCRIP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro56 of human MCRIP1 protein. Background Mapk-regulated corepressor-interacting protein 1 (MCRIP1) is a widely expressed protein substrate of ERK. MCRIP1 functions in regulating transcriptional repression of E-cadherin by the transcriptional corepressor CtBP. Unphosphorylated MCRIP1 binds to CtBP, preventing its transcriptional regulatory activity. When MCRIP1 is phosphorylated by ERK, CtIP is free to interact with the transcriptional repressor, ZEB1. The CtIP/ZEB1 complex then silences E-cadherin expression, promoting epithelial-mesenchymal transition (EMT), a process in tumor progression that leads to enhanced migration and invasion (1,2). Alternate Names DKFZp686A17165; FAM195B; family with sequence similarity 195, member B; GRAN2; Granulin-2; hypothetical protein LOC348262; MAPK regulated corepressor interacting protein 1; MAPK-regulated co-repressor interacting protein 1; Mapk-regulated corepressor-interacting protein 1; MCRI1; MCRIP; MCRIP1; MGC133184; Protein FAM195B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 11 Source/Isotype: Rabbit IgG