CST, 78148S, Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel

Assay Kit for studying IL7R mouse/CD4 mouse/L-Selectin mouse/CD3epsilon mouse/CD44/CD3G mouse/CD8A mouse in the research area. Product Usage Information All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding a membrane impermeable viability dye such as Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis. Gating strategy for observing naive, effector, and memory T cell subsets: If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Next, observe expression of CD44 vs. CD62L on either helper T cells or cytotoxic T cells. Naive T cells are CD44-CD62L+. Central memory T cells are CD44+CD62L+. Effector memory and effector cells are CD44+CD62L-. To distinguish effector memory and effector cells, observe expression of CD127/IL-7Rα on the CD44+CD62L- population. Effector memory cells are positive for expression of CD127/IL-7Rα. Effector cells are negative for expression of CD127/IL-7Rα. Naive and central memory T cells also express CD127/IL-7Rα. Storage Antibodies are supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. Store at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific "Best By" dates for each individual component. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Each antibody in the Mouse Naive/Effector/Memory T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein and detects epitopes within the extracellular domain. CD3 (17A2) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. CD44 (IM7) Rat Monoclonal Antibody (PE-Cy5 Species Reactivity: Mouse Source / Purification Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.