CST, 7825S, PathScan® Total VEGFR-2 Sandwich ELISA Antibody Pair

ELISA Antibody Pair for studying VEGFR2 in the research area. Storage Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C. Protocol Available protocols: ELISA+ Specificity / Sensitivity For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan Species Reactivity: Human Background Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells and angiogenesis (6-8). Alternate Names KDR; VEGF Receptor; vegfr; VEGFR-2 elisa; vegfr2 Specification REACTIVITY: H