CST, 78288S, CDCP1 (F3M9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CdcP1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, CDCP1 (F3M9G) Rabbit Monoclonal Antibody (CST #78288) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CDCP1 (F3M9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDCP1 protein. The full-length, glycosylated CDCP1 protein band is detected at 140 kDa, and a cleavage product of CDCP1 protein is detected at 70 kDa. Non-specific bands are detected between 80-85 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDCP1 protein. Background CUB domain-containing protein 1 (CDCP1, SIMA135) is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative hematopoietic stem cell marker (4,5). Alternate Names CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140, 70 Source/Isotype: Rabbit IgG