CST, 78319S, Streptavidin

Avidin and Biotin for studying in the research area. Storage Supplied in a lyophilized form that is stable for 12 months after receipt. Upon receipt, store at 4°C. Reconstitute with ddH 2 O and store at 4°C. Upon reconstitution, the product is in 20 mM KPi (pH 6.5). Avoid multiple freeze/thaw cycles . Specificity / Sensitivity Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4). Source / Purification Streptavidin is a protein that is derived from the bacteria . Background Streptavidin is a 53 kDa homotetramer isolated from for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate and detect biotinylated proteins (1,2).