CST, 78328SF, Claudin-5 (E5D9Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Claudin-5. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Claudin-5 (E5D9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #78328) is ready to ship. Product Usage Information This product is the carrier free version of product #66879. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 66879 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Claudin-5 (E5D9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total claudin-5 protein. Species reactivity for immunohistochemistry is human only. Non-specific staining was observed in skeletal muscle by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala202 of human claudin-5 protein. Background Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-5, highly expressed in brain and lung tissue, is a primary component of the tight junctions of blood-brain barrier endothelial cells. The blood-brain barrier regulates homeostatic and pathological conditions in the brain by limiting passage of material circulating to the brain. The blood-brain barrier can also impede the effectiveness of small molecule therapeutics (4). Claudin-5 is also thought to regulate tumor cell motility brain metastasis (5). Alternate Names androgen withdrawal and apoptosis induced protein RVP1-like; AWAL; BEC1; claudin 5; Claudin-5; claudin5; CLD5; CLDN5; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG