CST, 7834S, PathScan® Phospho-NF-kappaB p65 (Ser536) Sandwich ELISA Antibody Pair

ELISA Antibody Pair for studying NFkB-p65 (Ser536) phosphate in the research area. Product Usage Information Antibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily. Storage Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C. Protocol Available protocols: ELISA+ Specificity / Sensitivity For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan Species Reactivity: Human, Mouse Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Alternate Names NF-kB; NFkappaB; NFkB; nfκb p65; nuclear factor kappa B; p65 NF-kB; Rel; RelA Specification REACTIVITY: H M