CST, 78385S, PSMB10/MECL-1 Antibody

Polyclonal Antibody for studying PSMB10. Validated for Western Blotting. Highly specific and rigorously validated in-house, PSMB10/MECL-1 Antibody (CST #78385) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMB10/MECL-1 Antibody recognizes endogenous levels of total PSMB10/MECL-1 protein. This antibody does not cross-react with PSMB7/Z protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PSMB10/MECL-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades polyubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9). Proteasome subunit β type-10 (PSMB10, LMP10, MECL-1) is expressed as a proenzyme that is autocleaved to form a mature immunoproteasome core particle subunit (10). Like other immunoproteasome subunits, PSMB10/MECL-1 expression is induced by IFN-γ and subsequent replacement of constitutively expressed PSMB7/Z within the 20S proteasome proteolytic core particle facilitates processing and presentation of MHC class I-restricted peptide antigens on the cell surface (11-13). Alternate Names beta2i; LMP10; Low molecular mass protein 10; Macropain subunit MECl-1; MECL1; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome 20S subunit beta 10; proteasome catalytic subunit 2i; Proteasome MECl-1; proteasome subunit beta 10; proteasome subunit beta 7i; Proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit beta2i; proteasome subunit MECL1; PSB10; PSMB10 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 25, 29 SOURCE: Rabbit