CST, 78399S, MMP-8 (E1C4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MMP8. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-8 (E1C4L) Rabbit Monoclonal Antibody (CST #78399) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity MMP-8 (E1C4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MMP-8 protein by IHC-P and transfected levels of MMP-8 protein by Western blot. Non-specific staining was observed in human salivary gland by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MMP-8 protein. Background The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). MMP-8 is also known as Neutrophil Collagenase as it is stored in special granules in neutrophils. Like other MMPs, it also cleaves fibrillar collagens. It also is implicated in various cancers and is thought to be pro-tumorigenic (6). Alternate Names CLG1; collagenase 2; HNC; matrix metallopeptidase 8; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; MMP8; Neutrophil collagenase; PMN leukocyte collagenase; PMNL collagenase; PMNL-CL Specification REACTIVITY: H SENSITIVITY: Endogenous (IHC-P), Transfected (W) MW (kDa): 70 Source/Isotype: Rabbit IgG