CST, 78401S, FcR gamma (E6Y1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcER1G. Validated for WB,IP,IHC,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, FcR gamma (E6Y1A) Rabbit Monoclonal Antibody (CST #78401) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98492 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FcR gamma (E6Y1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcRγ protein. Non-specific staining was observed in normal human kidney and salivary gland by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FcRγ protein. Background FcRγ (Fc receptor gamma-chain, FcεRI gamma, High affinity immunoglobulin epsilon receptor subunit gamma) is a component of the IgE Fc receptor FcεRI, a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains (FcRγ) (1,2). FcεRI is expressed exclusively on basophils and mast cells and is involved in triggering allergic reactions (1-3). FcRγ also forms signaling complexes with other Fc receptors, including FcγRIA and FcγRIIIA. FcRγ shows high homology to the T cell receptor (TCR) ζ chain (4-6) and contains an immunoreceptor tyrosine-based activation motif (ITAM) which elicits a pro-inflammatory response (1-3). Alternate Names Fc epsilon receptor Ig; Fc fragment of IgE receptor Ig; Fc fragment of IgE, high affinity I, receptor for; Fc receptor gamma-chain; Fc-epsilon RI-gamma; FCER1G; FCERG; FceRI gamma; FCRG; FcRgamma; gamma polypeptide; High affinity immunoglobulin epsilon receptor subunit gamma; IgE Fc receptor subunit gamma; immunoglobulin E receptor, high affinity, gamma chain Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG