CST, 78454W2, Ki-67 (D3B5) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying KI-67. Highly specific and rigorously validated in-house, Ki-67 (D3B5) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #78454) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Ki-67 (D3B5) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein. Background Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4). Alternate Names antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; KI-67; KI67; KIA; marker of proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG