CST, 7854S, PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Antibody Pair

ELISA Antibody Pair for studying 4E-BP1 (Thr37/Thr46) phosphate in the research area. Storage Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C. Protocol Available protocols: ELISA+ Specificity / Sensitivity For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan Species Reactivity: Human, Mouse, Rat, Monkey Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Alternate Names 4e-bp; 4e-bp1; 4E-BP1 elisa; 4EBP-1 elisa; 4EBP1 elisa; cell based assay; cell-based assay; sandwich elisa; screening Specification REACTIVITY: H M R Mk