CST, 78749SF, Cathepsin B (D1C7Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cathepsin B. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Cathepsin B (D1C7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #78749) is ready to ship. Product Usage Information This product is the carrier free version of product #31718. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 31718 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cathepsin B (D1C7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total cathepsin B protein. This antibody detects the heavy chain subunit of cathepsin B. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the heavy chain subunit of human cathepsin B protein. Background Cathepsin B (CTSB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase (1,2). Cathepsin B is produced from a larger precursor form, pro-cathepsin B, which runs at approximately 44 kDa on SDS-PAGE, and is proteolytically processed and glycosylated to form a mature two-chain protein containing a heavy chain (running at 27 and 24 kDa) and a light chain (5 kDa). High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis (3), rheumatoid arthritis (4), and pancreatitis (5). While generally localized to lysosomes, in cancer alterations can lead to its secretion (6). Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis (7,8). Expression can correlate with poor prognosis for a variety of forms of cancer (9-13). This product detects a SARS-CoV-2-related target for research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic. Alternate Names amyloid precursor protein secretase; APP secretase; APPS; CATB; Cathepsin B; Cathepsin B heavy chain; Cathepsin B light chain; Cathepsin B1; CPSB; CTSB; cysteine protease; epididymis secretory sperm binding protein; RECEUP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 44, 27, 24 Source/Isotype: Rabbit IgG