CST, 78771S, FATP2/SLC27A2 (E8F8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FATP2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FATP2/SLC27A2 (E8F8O) Rabbit Monoclonal Antibody (CST #78771) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FATP2/SLC27A2 (E8F8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total FATP2/SLC27A2 protein. This antibody does not cross-react with FATP1/SLC27A1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val381 of human FATP2/SLC27A2 protein. Background Fatty acid transport protein 2 (FATP2), also known as solute carrier family 27 member 2 (SLC27A2), transports long-chain fatty acids into cells at the cell membrane. FATP2/SLC27A2 also functions as a very long-chain acyl-CoA synthetase in the endoplasmic reticulum to activate very long-chain fatty acids (1). Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are pathologically activated neutrophils that accumulate in many diseases. The immunosuppressive activity of PMN-MDSCs is critical in regulating immune responses in cancer. Studies show that FATP2/SLC27A2 expression is upregulated in PMN-MDSCs. FATP2/SLC27A2 mediates the suppressive activity of PMN-MDSCs by increasing the uptake of arachidonic acid and synthesis of prostaglandin E2. In addition, deletion of FATP2/SLC27A2 is shown to abolish the suppressive activity of PMN-MDSCs (2). These results suggest that targeting FATP2/SLC27A2 may increase anti-tumor immunity (2,3). Alternate Names ACSVL1; Arachidonate--CoA ligase; FACVL1; FATP-2; FATP2; Fatty acid transport protein 2; Fatty-acid-coenzyme A ligase, very long-chain 1; hFACVL1; HsT17226; Long-chain fatty acid transport protein 2; Long-chain-fatty-acid--CoA ligase; Phytanate--CoA ligase; S27A2; SLC27A2; solute carrier family 27 (fatty acid transporter), member 2; Solute carrier family 27 member 2; THCA-CoA ligase; Very long-chain acyl-CoA synthetase; very long-chain fatty-acid-coenzyme A ligase 1; Very long-chain-fatty-acid-CoA ligase; very-long-chain acyl-CoA synthetase; VLACS; VLCS Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG