CST, 78869SF, NF-kappaB1 p105/p50 (D7H5M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NFkB-p105. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, NF-kappaB1 p105/p50 (D7H5M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #78869) is ready to ship. Product Usage Information This product is the carrier free version of product #12540. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12540 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NF-kappaB1 p105/p50 (D7H5M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NF-κB1 p105/p50 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly415 of human NF-κB p105/p50 protein. Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50 (12,13). Alternate Names CVID12; DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; MGC54151; NF-kappa-B; NF-kappa-B1; NF-kappaB; NF-kappabeta; NF-kB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; NFKB1/p105; NFKB1/p50; nuclear factor kappa B subunit 1; nuclear factor kappa-B DNA binding subunit; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105; p50 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50 Active form. 120 Precursor Source/Isotype: Rabbit IgG