CST, 78923T, CD11b/ITGAM (E6E1M) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying CD11b/ITGAM mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CD11b/ITGAM (E6E1M) Horse Chimeric Monoclonal Antibody (CST #78923) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity CD11b/ITGAM (E6E1M) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total CD11b/ITGAM protein. Species Reactivity: Mouse Source / Purification This recombinant chimeric antibody is engineered from CD11b/ITGAM (E6E1M) Rabbit mAb #17800 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, CD11b/ITGAM (E6E1M) Rabbit mAb #17800, is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD11b/ITGAM protein. Background Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4). Alternate Names CD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; integrin alpha M; Integrin alpha-M; ITAM; Itgam; Leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; Mac-1; Mac-1 alpha; Mac-1a; MAC1; macrophage antigen alpha Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4