CST, 79004T, ISG20 (E6A2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ISG20. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ISG20 (E6A2Z) Rabbit Monoclonal Antibody (CST #79004) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ISG20 (E6A2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ISG20 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ISG20 protein. Background Interferon-stimulated 20 kDa protein (ISG20) is an exoribonuclease that functions as part of the host innate immune response to RNA-based viruses. Indeed, expression of ISG20 can be induced by type-I and type-II interferons following cellular exposure to RNA-based viruses (1). Mechanistically, ISG20 localizes to the nucleolus and is believed to inhibit the replication of RNA-based viruses, in part, by employing 3'-5' exonuclease activity toward viral RNAs (2-5). Alternate Names CD25; Estrogen-regulated transcript 45 protein; HEM45; interferon stimulated exonuclease gene 20; interferon stimulated exonuclease gene 20kDa; Interferon-stimulated gene 20 kDa protein; ISG20; Promyelocytic leukemia nuclear body-associated protein ISG20 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG