CST, 79030S, TIF1 alpha/TRIM24 (E9T3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIF1-alpha. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TIF1 alpha/TRIM24 (E9T3N) Rabbit Monoclonal Antibody (CST #79030) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity TIF1 alpha/TRIM24 (E9T3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIF1α/TRIM24 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein. Background TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs - an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17). Alternate Names E3 ubiquitin-protein ligase TRIM24; hTIF1; PTC6; RING finger protein 82; RING-type E3 ubiquitin transferase TIF1-alpha; RNF82; TF1A; TIF1; TIF1-alpha; TIF1A; TIF1ALPHA; Transcription intermediary factor 1-alpha; transcriptional intermediary factor 1; TRIM24; tripartite motif containing 24; tripartite motif protein 24; tripartite motif-containing 24; Tripartite motif-containing protein 24 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140, 160, 180 Source/Isotype: Rabbit IgG