CST, 79037SF, Aurora A (D3E4Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Aurora A. Validated for WB,IF,F,ELISA+. Highly specific and rigorously validated in-house, Aurora A (D3E4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #79037) is ready to ship. Product Usage Information This product is the carrier free version of product #14475. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14475 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Aurora A (D3E4Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Aurora A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro70 of human Aurora A protein. Background Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6). Alternate Names AIK; AIRK1; ARK-1; ARK1; AURA; AURKA; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; hARK1; IAK1; IPL1-related kinase; MGC34538; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK6; STK7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG