CST, 79083S, DPEP-1/MDP Antibody

Polyclonal Antibody for studying DPEP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, DPEP-1/MDP Antibody (CST #79083) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DPEP-1/MDP Antibody recognizes endogenous levels of total DPEP-1/MDP protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro312 of human DPEP-1/MDP protein. Antibodies are purified by peptide affinity chromatography. Background Dipeptidase-1 (DPEP-1, RDP, MDP) is a GPI-linked glycoprotein expressed in the epithelial brush border of the kidney epithelium and exists as a disulfide-linked homodimer (1). Research studies have shown that the enzymatic activity of DPEP-1 is involved in kidney metabolism of glutathione, leukotrienes, and β-lactam antibiotics (2,3). Independent of its enzymatic activity, DPEP-1 has been implicated in pathologic inflammation by acting as an adhesion receptor for neutrophils on endothelial cells within the lung (4). DPEP-1 has also been implicated in human cancer and numerous studies have demonstrated that expression of this enzyme is upregulated in colorectal cancer and promotes metastasis (5-7). Alternate Names Beta-lactamase; Dehydropeptidase-I; Dipeptidase 1; dipeptidase 1 (renal); DPEP1; hRDP; MBD1; MDP; Microsomal dipeptidase; RDP; Renal dipeptidase; testicular tissue protein Li 57 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 58 SOURCE: Rabbit