CST, 79109T, PAR1 (E9J9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAR-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PAR1 (E9J9L) Rabbit Monoclonal Antibody (CST #79109) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PAR1 (E9J9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR1 protein. This antibody recognizes a background band at 24 kDa of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp50 of human PAR1 protein. Background PAR1 (F2R) belongs to the PAR (Protease-Activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through amino terminal cleavage of the receptor by serine proteases such as thrombin, trypsin, or matrix metalloproteinases (1,2). Protease-mediated cleavage exposes the ‘tethered-ligand' fragment of the receptor; this binds to a second extracellular loop of the receptor and triggers receptor activation. Activated PAR1 couples to distinct G-protein complexes or arrestins, further relaying signals to downstream players (e.g., phospholipase C, Rho-kinase) (3-4). PAR1 function is necessary for normal physiological processes such as hemostasis, platelet activation, and thrombosis, whereas abnormal activation of PAR1 has been associated with inflammatory disorders, cardiovascular diseases, and metastasis (4-6). Alternate Names CF2R; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; coagulation factor II thrombin receptor; F2R; HTR; PAR-1; PAR1; protease-activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 47, 70-110 Source/Isotype: Rabbit IgG