CST, 79132S, IRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying IRF-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, IRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated) (CST #79132) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total IRF-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro261 of human IRF-1 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Alternate Names Interferon regulatory factor 1; IRF-1; IRF1; MAR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45-48 Source/Isotype: Rabbit IgG