CST, 79139T, Phospho-SHIP1 (Tyr865) (F3N9M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHIP1 (Tyr865) phosphate. Validated for WB,WB,IP,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SHIP1 (Tyr865) (F3N9M) Rabbit Monoclonal Antibody (CST #79139) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-SHIP1 (Tyr865) (F3N9M) Rabbit Monoclonal Antibody recognizes endogenous levels of SHIP1 protein only when phosphorylated at Tyr865 of human SHIP1, Tyr868 of mouse SHIP1, or Tyr867 of rat SHIP1. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr865 of human SHIP1 protein. Background SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1022 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). SHIP1 can also shuttle to the nucleus where it has a pro-proliferative function. Nuclear accumulation of SHIP1 has been linked to acute myeloid leukemia (AML). Phosphorylation of SHIP1 at Tyr865, as well as, Tyr1022 decreases nuclear localization, shuttling the protein to the cell membrane where it has an inhibitory effect on PI3K signaling (7). Alternate Names hp51CN; Inositol polyphosphate-5-phosphatase D; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; MGC104855; MGC142140; MGC142142; p150Ship; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1; SH2 containing inositol phosphatase, isoform b; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5'-phosphatase 1; SHIP; SHIP-1; SHIP1; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II; SIP-145 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG